Fluorescent labling methods by rhodamine B isothiocyanate in cellulose Materials

Document Type : Research Paper

Authors

1 Wood and paper Science and Technology Department, Tarbiat Modares, university

2 Wood and Paper Science and Technology Department, Natural Resources Faculty, Tarbiat Modares University, Noor, Mazandaran,Iran

10.22034/ijwp.2023.703799

Abstract

Staining with fluorescent materials is one of the widely used methods in studying and locating cellulosic materials. In this study, the staining of cellulosic materials was investigated by two approaches as direct (direct addition of fluorescent labelling agent) and indirect (adding fluorescent label after creating an anchor) methods; then the staining results were compared. The results showed that although imaging was possible in both methods, indirect staining provided relatively more uniform and effective image results during this investigation. The results showed although fluorescent imaging was possible following both methods, the use of indirect staining method provided relatively better and cleaner image results during this investigation. Moreover, the effect of each staining method on the surface charge property (zeta potential of nanofibers) and wet-end interactions (via freeness testing as an indicator) were studied. The results showed that the (negative) zeta potential of cellulose material became more negative as a result of staining following both staining methods. It was also observed that the staining of the cellulose material by both methods did not affect the freeness as an indicator.

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References

[1] Horobin, R. and Kiernan, J., eds., 2002. Conn's biological stains: A handbook of dyes, stains and fluorochromes for use in biology and medicine, Taylor & Francis.
[2] Mycek, M.A. and Pogue, B.W., 2003. Handbook of biomedical fluorescence, CRC Press, Boca Raton, FL.
[3] Hubbe, M.A., Chandra, R. P., Dogu, D. and Velzen, S.T.J., 2019. Analytical staining review. BioResources, 14(3): 7387-7464.
[4] Jin, D. and Piper, J.A., 2011. Time-gated luminescence microscopy allowing direct visual inspection of lanthanide-stained microorganisms in background-free condition. Anal. Chem., 83(6): 2294–2300.
[5] Hagen, G.M., Bendesky, J., Machado, R., Nguyen, T.A., Kumar, T. and Ventura, J., 2021. Fluorescence microscopy datasets for training deep neural networks. GigaScience, 10(5): 1-6 (giab032).
[6] Dong, S.P. and Roman, M., 2007. Fluorescently labeled cellulose nanocrystals for bio-imaging applications. J. Am. Chem. Soc., 129, 13810-13811.
[7] Ding, Q., Zeng, J., Wang, B., Gao, W., Chen, K., Yuan, Z., Xu, J. and Tang, D., 2018. Effect of retention rate of fluorescent cellulose nanofibrils on paper properties and structure. Carbohydr. Polymers, 186: 73-81.
[8] Botelho, D.R., Vieiri, F., 2001. Photonic and electronic spectroscopies for the characterization of organic surfaces and organic molecules adsorbed on surfaces, in: Handbook of surface and interface analysis and properties, Academic Press.
[9] Rohrling, J., Potthast, A., Rosenau, T., Lange, T., Ebner, G., Sixta, H. and Kosma, P., 2002. A novel method for the determination of carbonyl groups in cellulosics by fluorescence labeling. 1. method development. Biomacromolecules, 3(5): 959-968.
[10] Hobisch, M.A., Bossu, J., Mandlez, D., Spirk, S., Eckhart, R. and Bauer, W., 2019. Localization of cellulose fines in paper via fluorescent labeling. Cellulose, 26, 6933-6942.
[11] Wang, S., Gao, W., Chen, K., Zeng, J., Xu, J. and Wang, B., 2018. An effective method for determining the retention and distribution of cellulose nanofibrils in paper handsheets by dye labeling. TAPPI Journal, 17(3): 157–164.
[12] Whipple, W.L. and Maltesh, C., 2000. Visualizing flocculation and adsorbtion processes in papermaking using fluorescence microscopy. Langmuir, 16(7): 3124-3132.
[13] Huang, L., He, L., Gao, W., Zeng, J., Wang, B., Xu, J. and Chen, K., 2020. Distribution analysis of cellulose nanofibrils in paper handsheets: dyelabeled method, Carbohydrate Polymers, 239, 116-226.
[14] Orelma, H., Filpponen, I., Johansson, L. S., Osterberg, M., Rojas, O. J. and Laine, J., 2012. Surface functionalized nanofibrillar cellulose (NFC) film as a platform for immunoassays and diagnistics, Biointerphases, 61(7): 1-4.
[15] William E. Scott, 1996. Principles of Wet End Chemistry, Tappi Pr, 185 pages.
[16] TAPPI T205 sp-02, 2007. Forming handsheets for physical tests of pulp.
[17] TAPPI T227 om-04, 2007. Freeness of pulp (Canadian standard method).
[18] Yousefhashemi, S. M., Khosravani A. and Yousefi., H., 2019. The effect of addition of lignocellulose nanofiber produced from old corrugated container pulp on recycled paperboard properties, Iranian Journal of Wood and Paper Industries, 9(4): 575-584. (In Persian)
[19] Yousefhashemi, S. M., Khosravani, A. and Yousefi H., 2019.  Isolation of lignocellulose nanofiber from recycled old corrugated container and its interaction with cationic starch-nanosilica combination to make paperboard, Cellulose, 26: 7207-7221.
[20] Najideh, R., Rahmaninia, M. and Khosravani A., 2021. Cellulose nanofibers made from waste printing and writing papers and its effect on the properties of recycled paper, Iranian Journal of Wood and Paper Industries, 12(2): 185-194. (In Persian)
Iranian Journal of Wood and Paper Industries
Volume 13, Issue 4
March 2023
Pages 405-417

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