Iranian Journal of Wood and Paper Industries

Iranian Journal of Wood and Paper Industries

Influence of Xylanase on the Viscosity and Mechanical Properties of Bagasse Pulp in ECF Bleaching Sequences

Document Type : Research Paper

Authors
Pejman Rezayati Charani 1
Fatemeh Qaisari Asl 2
MohammadHadi Moradian 3
Ladan Poursartip 3
1 Wood and Paper Industries, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
2 Graduated Master of pulp and paper technology, Department of cellulose technology engineering, natural resources faculty, Behbahan Khatam Alanbia university of technology, Behbahan
3 Assistant Prof., Department of Cellulose Technology Engineering, Natural Resources Faculty, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran
10.22034/ijwp.2024.2038168.1679
Abstract
Problem definition and objectives: Treatments are carried out in different ways to dissolve and remove colored substances, mainly lignin, to prepare paper pulp from different lignocellulosic sources. Treatment methods, whether in combination or individually, can significantly impact the strength of the cellulose fibers used in papermaking. The bleaching treatment of paper pulp, which is usually used after pulping, is always associated with damage to the structure of paper pulp. In this regard, it is tried to use selective methods in bleaching. Determining the viscosity of pulp fibers is a measure to evaluate their strength in papermaking. The viscosity of pulp fibers shows the amount of chemical damage to the fibers through indirect measurement of the cellulose chain polymerization degree in the fibers. The viscosity of paper pulp is a measure to evaluate its strength in papermaking. This study investigated the effect of the xylanase enzyme on maintaining the viscosity of unbleached soda bagasse pulp prepared from Pars Paper Industries through ECF bleaching using pulp preparation methods with limited viscosity loss.
Methodology: Unbleached bagasse pulp with 5% consistency was treated using xylanase enzyme at three levels (2, 5, and 10 U/g) and a pH of 5.5 for 60 minutes. Chemical bleaching with the sequence XE1DE2 was done at 10% pulp consistency and 70 and 60 degrees Celsius for stages D and E, respectively. The amount of active chlorine in both treatments was considered 3% based on the initial kappa number of the pulp. Properties of the pulp and handsheets, including pulp yield and viscosity, in addition to grammage, thickness, density, degree of brightness, fold resistance, and tensile strength of handsheets, were measured.
Results: Results showed that the yield of pulps decreased with increasing enzyme consumption and the use of more chemical bleaching steps. Xylanase treatment up to 2U level can help maintain the viscosity of pulp, but consumption of 5U and 10U severely reduces the viscosity of the final pulps. Xylanase treatment up to 2U can also increase pulp brightness during chemical bleaching. However, the higher consumption of xylanase will not affect the final paper's brightness but preserve its strength after bleaching, which showed itself in the evaluation of folding resistance and tensile strength. In addition, using xylanase treatment reduced the chlorine dioxide consumption to achieve a certain degree of brightness, which, regarding this factor, the consumption of more enzymes in a relatively linear manner reduced the consumption of chlorine dioxide in the bleaching stage.
Conclusion: In general, the moderate use of xylanase can be introduced at the level of 2U according to the information of this research, which leads to a reduction in the consumption of bleaching chemicals and can be environmentally significant. However, using more xylanases not only does not permanently increase the brightness but also causes damage to the mechanical resistance of the paper. In general, the usage of 2U xylanase is recommended for the bleaching of brown bagasse soda pulp.
Keywords
Xylanase
Soda pulping
Bagasse
Pulp viscosity
Bleaching

Subjects

[1] Abd El-Sayed, E.S., El-Sakhawy, M. and El-Sakhawy, M.A.-M., 2020. Non-wood fibers as raw material for pulp and paper industry. Nordic Pulp & Paper Research Journal,35, pp. 215–230.
[2]  Azizi Mossello, A., Savari, A. and Rezayati Charani, P., 2019. The Effect of Protexin Enzyme for Bagasse Storage on Pulp and Paper Properties. Iranian Journal of Wood and Paper Industries, 10(3), pp. 457–468. (In Persian).
[3]  Santos, R.B., Jameel, H., Chang, H.-M. and Hart, P.W., 2013. Impact of Lignin and Carbohydrate Chemical Structures on Kraft Pulping Process and Biofuel Production. TAPPI Journal, 12, pp. 23–31.
[4]  Wang, X., Liu, Y., Pu, J., Qin, C., Yao, S., Wang, S. and Liang, C., 2024. A comparative study on the structure of lignin-carbohydrate complexes in alkali-soluble hemicellulose from bamboo (Bambusa chungii) fibers and parenchyma cells. Industrial Crops and Products Journal, 210;118061. DOI: 10.1016/j.indcrop.2024.118061
[5]  Roberts, J.C., 2007. The chemistry of paper. Royal Society of Chemistry.
[6]  Lan, X., Fu, S., Song, J., Leu, S., Shen, J., Kong, Y., Kang, S., Yuan, X. and Liu, H., 2024. Structural changes of hemicellulose during pulping process and its interaction with nanocellulose. International Journal of Biological Macromolecules, 255, 127772. DOI: 10.1016/J.IJBIOMAC.2023.127772.
[7]  Mboowa, D., 2024. A review of the traditional pulping methods and the recent improvements in the pulping processes. Biomass Conversion and Biorefinery, 14(1), pp. 1–12. DOI: 10.1007/s13399-020-01243-6.
[8]  Zhang, L., Yu, D., Chen, Y. and Wu, C., 2024. Effect of Urea/choline chloride treatment on removing hemicellulose during alkali extraction in the preparation of high-purity dissolving pulps. Industrial Crops and Products Journal, 222, 119709. DOI: 10.1016/J.INDCROP.2024.119709.
[9]  Cuebas‐Irizarry, M.F. and Grunden, A.M., 2024. Streptomyces spp. as biocatalyst sources in pulp and paper and textile industries: Biodegradation, bioconversion and valorization of waste. Microbial Biotechnology, 17(1), p. e14258. DOI: 10.1111/1751-7915.14258.
[11]         Moradian Gilan, K., hedjazi, sahab, Abdolkhani, A. and Sixta, H., 2019. Using of xylanase and cold caustic extraction to remove hemicellulose from bagasse bleached pulp for dissolving pulp production. Iranian Journal of Wood and Paper Industries, 10(1), pp. 1–9. (In Persian).
 [12]        Alifia, K.C.H., Setiadi, T., Boopathy, R., Risdianto, H., Irfan, M. and Hidayatullah, I.M., 2023. Bio-bleaching agents used for paper and pulp produced from the valorization of corncob, wheat straw, and bagasse. In: Chemical Substitutes from Agricultural and Industrial By-Products: Bioconversion, Bioprocessing, and Biorefining. DOI: 10.1002/9783527841141.ch8.
[13]         Dhiman, S.S., Garg, G., Mahajan, R., Garg, N. and Sharma, J., 2009. ‘Single lay out’and ‘mixed lay out’enzymatic processes for bio-bleaching of kraft pulp. Bioresource Technology, 100(20), pp. 4736–4741. DOI: 10.1016/j.biortech.2009.04.059
[14]         Walia, A., Guleria, S., Mehta, P., Chauhan, A. and Parkash, J., 2017. Microbial xylanases and their industrial application in pulp and paper biobleaching: a review. 3 Biotech, 7(11), pp. 1–12. DOI: 10.1007/s13205-016-0584-6.
[15]         Kaur, G., Kaur, P., Kaur, J., Singla, D. and Taggar, M.S., 2024. Xylanase, xylooligosaccharide and xylitol production from lignocellulosic biomass: Exploring biovalorization of xylan from a sustainable biorefinery perspective. Industrial Crops and Products Journal, 215, 118610.
[16]         Deshpande, R., Sundvall, L., Grundberg, H., Lawoko, M. and Henriksson, G., 2020. Lignin carbohydrate complex studies during kraft pulping for producing paper grade pulp from birch. TAPPI Journal, 19(9), pp. 447–460.
[17]         Moon, R.J., Martini, A., Nairn, J., Simonsen, J. and Youngblood, J., 2011. Cellulose nanomaterials review: Structure, properties and nanocomposites. Chemical Society Reviews, 40(7). DOI: 10.1039/c0cs00108b.
[18]         Beg, Q.K., Bhushan, B., Kapoor, M. and Hoondal, G.S., 2000. Enhanced production of a thermostable xylanase from Streptomyces sp. QG-11-3 and its application in biobleaching of eucalyptus kraft pulp. Enzyme and Microbial Technology, 27(7), pp. 459–466. DOI: 10.1016/S0141-0229(00)00231-3.
[19]         Matos, J.M.S., Evtuguin, D. V, de Sousa, A.P.M. and Carvalho, M.G.V.S., 2024. Xylanase treatment of eucalypt kraft pulps: effect of carryover. Applied Microbiology and Biotechnology, 108(1), p. 210. DOI: 10.1007/s00253-024-13027-3.
[20]         Gangwar, A.K., Prakash, N.T. and Prakash, R., 2014. Applicability of microbial xylanases in paper pulp bleaching: a review. BioResources, 9(2). pp. 3733-3753.
[21]         Van der Brught, T., Tolan, J. and Thibault, L., 2002. US kraft mills lead in xylanase implementation. In: 2002 TAPPI Fall Technical Conference and Trade Fair.
[22]         Schaubeder, J.B., Spirk, S., Fliri, L., Orzan, E., Biegler, V., Palasingh, C., Selinger, J., Bakhshi, A., Bauer, W., Hirn, U. and Nypelö, T., 2024. Role of intrinsic and extrinsic xylan in softwood kraft pulp fiber networks. Carbohydrate Polymers, 323, p.121371. DOI: 10.1016/j.carbpol.2023.121371.
[23]         Kulkarni, N. and Rao, M., 1996. Application of xylanase from alkaliphilic thermophilic Bacillus sp. NCIM 59 in biobleaching of bagasse pulp. Journal of Biotechnology, 51(2). DOI: 10.1016/0168-1656(96)01616-1.
[24]         Jeffries, T.W., Davis, M., Rosin, B. and Landucci, L.L., 1998. Mechanisms for kappa reduction and color removal by xylanases. Enzyme, 4, pp. 2–319.
[25]         Bajpai, P., Bajpai, P.K., Kondo, R., Bajpai, P., Bajpai, P.K. and Kondo, R., 1999. Pulp Bleaching with xylanases. Biotechnology for Environmental Protection in the Pulp and Paper Industry, Springer Berlin, Heidelberg, pp. 49–64. DOI: 10.1007/978-3-642-60136-1.
[26]         Matos, J.M.S., Evtuguin, D. V, de Sousa, A.P.M. and Carvalho, M.G.V.S., 2024. Xylanase treatment of eucalypt kraft pulps: effect of carryover. Applied Microbiology and Biotechnology, 108(1), p. 210. DOI: 10.1007/s00253-024-13027-3.
[27]         Roncero, M.B., Torres, A.L., Colom, J.F. and Vidal, T., 2004. The effect of xylanase on lignocellulosic components during the bleaching of wood pulps. Bioresource Technology, 96(1), pp. 21–30.  DOI: 10.1016/j.biortech.2004.03.003.
[28]         Roncero, M.B., Torres, A.L., Colom, J.F. and Vidal, T., 2003. TCF bleaching of wheat straw pulp using ozone and xylanase. Part A: Paper quality assessment. Bioresource Technology, 87(3), pp. 305–314. DOI: 10.1016/S0960-8524(02)00224-9.
[29]         Kuhad, R.C., Rapoport, A., Kumar, Vinod, Singh, D., Kumar, Vijay, Tiwari, S.K., Ahlawat, S., Singh, B., 2024. Biological pretreatment of lignocellulosic biomass: An environment-benign and sustainable approach for conversion of solid waste
into value-added products. Critical Reviews in Environmental Science and Technology, 54, 771–796. DOI: 10.1080/10643389.2023.2277670.
[30]         Madlala, A.M., Bissoon, S., Singh, S. and Christov, L., 2001. Xylanase-induced reduction of chlorine dioxide consumption during elemental chlorine-free bleaching of different pulp types. Biotechnology Letters, 23(5), pp. 345–351. DOI: 10.1023/A:1005693205016.
[31]         Paice, M.G., Bernier, R. and Jurasek, L., 1988. Viscosity‐enhancing bleaching of hardwood kraft pulp with xylanase from a cloned gene. Biotechnology and Bioengineering, 32(2). DOI: 10.1002/bit.260320214.
[32]         Vinod Kumar, N., Rani, M.E., Gunaseeli, R. and Kannan, N.D., 2018. Paper pulp modification and deinking efficiency of cellulase-xylanase complex from Escherichia coli SD5. International Journal of Biological Macromolecules 111. DOI: 10.1016/j.ijbiomac.2017.12.126.
[33]         Przybysz Buzała, K., Kalinowska, H., Borkowski, J. and Przybysz, P., 2018. Effect of xylanases on refining process and kraft pulp properties. Cellulose, 25(2), pp. 1319–1328. DOI: 10.1007/s10570-017-1609-
Iranian Journal of Wood and Paper Industries
Volume 16, Issue 1
Winter 2025
Pages 15-28