Investigation of the Amount of Tannic Acid in Bark Oak (Quercus castanifolia)

Document Type : Research Paper

Authors

Abstract

In order to perform a qualitative and quantitative investigation on tannic acid content in Oak bark, the bark samples were collected from Educational Forest of Gorgan Agricatural Science and Natural Resources University. Solvents were used in laboratory to extract tannin and were considered as variables. Regarding diversity of bark components, Tappi Test Method (T264-om-88) was used to determin extractives contest. Twenty grams dry material was placed in a soxhelt. Extracted material with hot water, methanol and water- methanol (1:1) solvent were 25±2, 20±2 and 18±2 percents respectively. Tannic acid existance in extracts was determined using High Performance Liquid Choromatography (HPLC) and types of bonds were determined using FT-IR. Results showed that water-methanol solvent was the best solvent for extracting tannin.the amount of extracted tannic acid in maximum wavelength of 250 nm and using gradient system, in solvent water-methanol, methanol and hot water solvent were 70±2, 30±2 and 5±2 precents, respectively. Amount of tannin in the bark using solvent water-methanol was determined to be 14 perecents that was the highest amount.

Keywords

References

- ترکمن، ج.، دوست حسینی، ک. و میرشکرایی، س.ا. 1382. بررسی تانن پوست درختان توسکا وبلوط به روش اسپکتروفتومتری. مجله منابع طبیعی ایران. جلد 56. شماره 3. 280-271.
2- خلخالی، ب. 1323. بررسی و تعیین مقدار تانن در انار ساوه. دانشگاه تهران. دانشکده داروسازی. پایان نامه برای دریافت درجه دکتری.
3- دوست حسینی، ک. 1380. فناوری تولید و کاربرد صفحات فشرده چوبی. انتشارات دانشگاه تهران. 648 ص.
4- متوسلیان، م. 1359. اندازه گیری مواد مستخرجه دانه بلوط برای ارزش غذایی و دارویی آن. دانشگاه تهران. دانشکده داروسازی. پایان نامه برای دریافت درجه دکتری.
5- میرشکرایی، ا. 1371. در ترجمه مبانی و کاربردهای شیمی چوب، شوستروم. ا. (مؤلف). مرکز نشر دانشگاهی تهران. 257ص.
6- موثق، ب. 1380. در ترجمه نگرشی بر طیف سنجی، پاویا، د.، لمیمن، گ و کریز، ج. (مؤلفین). انتشارات علمی و فنی. 650 ص.
7- Babayi, H., Kolo, I., Okogun, J.I and Ijahoj, J. 2004. The anti microbial activites of methanolic extracts of Eucalyptus camadulensis and Terminalia catappa against some pathogenic micro organisms. Biokemistri. 16(2):106-111
8- Belleau, G and Dadic, M. 1979. Determination of Tannic acid in Beer by High Performance Liquid Choromatograpy. ASBC. Journal 37(4):175-179
9-lee, W. j., and Lan, W. C. 2006. Properties of resorcinol–tannin–formaldehyde copolymer resins prepared from the bark extracts of Taiwan acacia and China fir.  J bioresource technology. 97. 257-264
10- Nishmoura, H., Nonaka, G.I and Nishoka, I. 1986. Scyllo-Quercus gallates and Hexahydroxy diphenoates from Quercus stenophylla. Faculty of pharmaceutical science.kyusha university Japan.
11- Pansera, M.R., Antoniolob, G., Atti-santos. A.C., Rossato, M., Atti-serafini, Land Cassel, E. 2004. Extraction of Tannin by Acacia mearnsii with Supercritical fluids. Brazilian Archives of Biology and Technology. 47(6):995-998
12- Scalbert, A., Monties, B and Faure, J.M. 1988. Poly phenols of Quercus Rober Adult tree and in vitro grown calli and shoots.Randoeuvreles-nancy cedex France.
13- Sowunmi.S.,Ebewele.R.O.,Peters.O.,and Conner.A.H.2000.Differential scanning calorimetry of hydrolysed mangrove Tannin. Polymer International. 49:574-578
14- Odenyo, A. A. and Osuji, P. O. 1998. Tannin-tolerant ruminal bacteria from East African rumimants. Can. J. Microbiol. 44: 905-909.
15- Onifade, K.R. 2001. Production of Tannin from the bark of Eucalyptus camadulensis. Au Jurnal of technology.5 (2)457-464.
16-Turley, D.J., Kelly, M. T. and Smyth, M. R. 1990. High- performance liquid chromatographic method for the comparison of tanning capacity of tannic acid batches used in the manufacture of pregnancy testing kits. J Chromatogr. 513: 263-269.
17-www.jordiflp.com
18- Yazaki, Y. Collins, P. J. and Iwashina, T. 1993. Extractves from Blackbutt (Eucalyptus Pilularis) Wood which Affect Gluebond Quality of Phenolic Resins. Holzforschung.47(5): 412-418.
 
 
Iranian Journal of Wood and Paper Industries
Volume 1, Issue 1 - Serial Number 1
January 2011
Pages 27-35

Files

Share

How to cite

Statistics